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KMID : 0545120150250060814
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Journal of Microbiology and Biotechnology
2015 Volume.25 No. 6 p.814 ~ p.819
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A Substrate Fed-Batch Biphasic Catalysis Process for the Production of Natural Crosslinking Agent Genipin with Fusarium solani ACCC 36223
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Yuyao Zhu
Botao Zhao
Xiaode Huang
Bin Chen
Hua Qian
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Abstract
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The natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. Because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. In this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. A 10 L ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying genipin yield. With Fusarium solani ACCC 36223, 15.7 g/l genipin in the ethyl acetate phase was obtained, corresponding to space-time yields of 0.654 g l-1 h-1.
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KEYWORD
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biphasic bicatalysis, geniposide, genipin, Fusarium solani ACCC 36223
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